Testing, Verification and Analysis

Biological Testing

Microorganisms play essential roles in maintaining both human society and the environment. We have practical methods in place to confirm the existence in water of microorganisms contributing to environmental purification as well as to carry out biological assessments to determine the hazardous substance content of water.

In step with the enhancement of living standards, the volume of chemical substances being used to achieve and maintain such standards has also grown and there are fears this will impact the environment in unexpected ways. To address this problem, we are engaged in biological testing aimed at comprehensively assessing the behavior of multiple chemical substances. Our testing methods are also capable of predicting the environmental impact of as yet unregulated substances. Furthermore, we are exploring how to better utilize microorganisms in environmental purification via the use of gene analysis technologies. In addition to engaging in testing, we are striving to improve the quality of effluent from our testing facilities as part of our ongoing development efforts aimed at creating technologies to support people’s well-being and the earth’s environment.

New Effluent Management Method Employing Biological Responses

Employing algae, crustacean, fish and other aquatic organisms, this technology enables the clear assessment of effluent safety via the evaluation of toxic impact on effluent and the comprehensive analysis of other chemical factors. Biological assessment of this kind is legally mandatory in many countries. Japan’s Ministry of the Environment also encourages the use of this method. Drawing on our expertise as a water treatment equipment manufacturer, we are using this method to help improve the quality of effluent. The use of this technology is helping us acquire an increasing breadth of findings that would have otherwise been unnoticed if we use only chemical analysis.

1. Short-Term Toxicity Test on Embryonic through Larval Stage Fish (Zebrafish)

Targeting zebrafish, this test is aimed at evaluating the impact of constant exposure to effluent over the course of growth from a zygote to larval fish.


2. Daphnia Reproduction Test (Ceriodaphnia Dubia)

Daphnia is positioned as a primary consumer in the aquatic food chain. This test evaluates the impact of effluent exposure on the growth and reproduction of daphnia.

Ceriodaphnia dubia

3.Algae Growth Inhibition Test (Pseudokirchneriella subcapitata)

The alga Pseudokirchneriella subcapitata is positioned as a producer in the aquatic food chain. This test evaluates the impact of effluent exposure on its growth and reproduction.

Pseudokirchneriella subcapitata
Pseudokirchneriella subcapitata

Development Background

In the course of performing chemical analysis, I began to think that there must be better methods for multilaterally evaluating effluent and learned about biological testing at a seminar hosted by the Ministry of the Environment. My interest in biology led me to want to incorporate this testing technology into Kobelco Eco-Solutions’ repertoire. Biological testing involves handling organisms and thus requires high reliability. With this in mind, my team acquired Good Laboratory Practice (GLP) certification, an international standard for laboratory testing using algae, in 2017. In Japan, we are the 10th organization to be GLP certified and the first among water treatment equipment manufacturers. Although biological testing of this kind is popular overseas, only a handful of tests have been carried out domestically. Accordingly, I have made a daily habit of studying relevant papers published by overseas researchers while engaging in development.

Wastewater Treatment Process Optimization Using the Molecular Biological Analysis Method

Employing gene analysis technologies, we are engaged in the analysis of microorganisms that play key roles in environmental purification, exploring better ways to realize their potential. The principal target of this analysis is microorganisms inhabiting activated sludge, which is used in sewage and wastewater treatment, with the aim of helping stabilize and optimize treatment processes.

1. Quantitative Analysis of Specific Microorganisms

We use a real-time PCR method that boasts high accuracy and sensitivity, to quantitatively analyze the existence of gene of targeted microorganisms in a specimen.
As the performance of wastewater treatment equipment largely hinges on these microorganisms, findings from periodic analyses are greatly useful in terms of maintaining and managing such equipment.

Real-time PCR equipment
(LightCycler® 480II developed by Roche)

2. Comprehensive Microbial Community Analysis (Next-Generation Sequence Analysis)

Employing cutting-edge genome sequence analysis technologies, this method comprehensively analyzes the microorganism composition of a specimen. This method enables statistical analysis—assuming differences between microbial communities inhabiting treatment facilities in different locations, seasonal and other variable conditions—aimed at determining the types of microorganism that significantly affect treatment performance.

Flows of the utilization of comprehensive microbial community analysis
Flows of the utilization of comprehensive microbial community analysis

3.RFLP Analysis (Patterning Analysis of the Whole Microbial Community)

The RFLP is a method that is used to determine human parentage as well as to identify crop varieties. Employing this method, we analyze the patterns of microbial communities inhabiting in a specimen. The RFLP analysis of periodically sampled specimens enables us to quickly estimate whether changes in the status of microbial communities took place.

RFLP analysis
(An example of microbial community patterns)

Development Background

Relationships between the number and types of microorganisms and the performance of wastewater treatment equipment vary by the content of effluent being treated. Because of this, actual analysis to determine such relationships necessarily requires detailed data collected from each treatment site. Therefore, we carry out data analysis with the help of those who handle the maintenance and management of wastewater treatment facilities as we are dependent on a variety of valuable input only they can contribute. We are thus striving to identify types of microorganisms that serve as benchmarks for optimal facility maintenance and management.

Environmental Analysis

With the aim of helping protect a sound environment and human well-being, we will employ our robust technological capabilities to support the pursuit of new research and development themes. In this way, we will contribute to environmental preservation and the development of a future society.

Tap Water and Wastewater

  • Tap water quality inspection
  • Water quality analysis for industry
  • Sewage and sludge analysis
  • Analysis of items under environmental standards
Gas chromatograph mass spectrometer
Gas chromatograph mass spectrometer

Waste treatment

  • Inspection of industrial waste (PCBs, metal, organic substances, etc.)
Devices used for ion chromatography
Devices used for ion chromatography


  • Measurement of PCB content in insulation oil
  • PCB measurement of waste with low-concentration PCB content
Equipment for preprocessing insulating oil for PCB measurement
Equipment for preprocessing insulating oil for PCB measurement


  • Registered as a Measurement Certification Business: Registration No. 16 (Hyogo Prefecture; density measurement)
  • Registered as a Tap Water Quality Inspection Organization: Registration No. 132
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